You've got $24861 on line 17 of Schedule A but program is only taking Std deduction of $24800.

Have you by chance checked the box on Federal Information Sheet Part X - Use Std Deduction even if less than Itemized?         

Is it using $24,800 std deduction plus $300 charitable contributions?

This is what is happening.  The program is correctly calculating that a $24,800 SD PLUS a $300 above the line charitable contribution deduction is better taxwise than a $24,861 itemized deduction.

assuming there are charitable contributions

Be careful - if you are also filing a state return where the standard deduction is very small (like Maryland) if you are that close you might want to force itemizing thereby giving you more itemized deductions for the state. That number is just too close to not see which is more beneficial.

Pro series did a good job on this , I give them credit...

Give yourself credit for figuring it out.